Toxicology International (Formerly Indian Journal of Toxicology)

K. S. Gopi ¹, A. Gopala Reddy ¹, K. Jyothi ¹, B. Anil Kumar ¹

Affiliations
1 Department of Pharmacology and Toxicology, College of Veterinary Science, Hyderabad - 500030, Andhra Pradesh, IN

Abstract

Experimental study was conducted to evaluate the hepato- and renoprotective effect of silymarin and Terminalia chebula against experimentally-induced acetaminophen (APAP) toxicity in rats. Oral administration of APAP @ 500 mg/kg for 1 to 3 days to all the four groups (six rats in each) resulted in significant elevation of serum triglycerides, total cholesterol, blood urea nitrogen, serum creatinine, and aspartate transaminase activity. Posttreatment with silymarin @ 25 mg/kg and T. chebula 125 mg/kg in groups 2 and 3 and their combination to group 4 from day 4 to 14 has significantly reversed the alterations of above said markers and offered better protection. The results of the study enunciated that silymarin and T. chebula exhibit good hepato- and nephro-protection against APAP toxicity.

Keywords

Acetaminophen, hepatotoxicity, nephrotoxicity, silymarin, Terminalia chebula

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K. Jyothi , A. Gopala Reddy , Gopi K. S. , B. Anil Kumar , G. Dilip Reddy

Abstract

A study was conducted to evaluate the protective effect of N-acetyl cysteine (NAC) against experimentally-induced renal toxicity due to cyclophosphamide (CYP) in rats. Three groups (2, 3 and 4) of female Wistar Kyoto rats with 6 animals in each group were administered with CYP @ 40 mg/kg bwt on 1^st, 3^rd, 5^th and 7^th day by intraperitoneal route. Group 2 remained as toxic control. Group 3 received NAC from 1^st to 14^th day @ 0.2% of feed and group 4 was treated with NAC from 8^th to 14^th day. Group 1 was maintained on basal diet for 14 days. The study revealed a significant (P < 0.05) increase in the concentration of serum creatinine, blood urea nitrogen, thiobarbituric acid reacting substances (TBARS) and protein carbonyls, and a decrease in the reduced glutathione (GSH) concentration of kidney. These results are suggestive of CYP-induced oxidative stress resulting in renal damage. Feeding of NAC could not offer protection against CYPinduced renal damage in this study.

Keywords

Cyclophosphamide (CYP), N-acetyl cysteine (NAC), Oxidative stress

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